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Biotecnologie


Department: Dipartimento di Agraria
Location and Contacts: c/o Dipartimento Agraria
Feo di Vito
89122 Reggio Calabria
Tel. +39 0965 801255
Fax +39 0965 312827
Area (mq): 120
Rooms: 2
Director: Leonardo Schena
Mail: Mail
Technical Responsible: Antonio Tiberini
Full Professors: Gaetano Magnano Di San Lio (AGR/12)
Associate Professors: Giovanni Enrico Agosteo (AGR/12)
Giuliana Renata Albanese (AGR/12)
Leonardo Schena (AGR/12)
Vincenzo Palmeri (AGR/11)
Rita Marullo (AGR/11)
Rocco Zappia (AGR/03)
Researchers: Maria Giulia Li Destri Nicosia (AGR/12)
Gregorio Antonio Maria Gullo (AGR/03)
Valentino Branca (AGR/03)
Rocco Mafrica (AGR/03)
Antonio Tiberini (AGR/12)
PhD graduates: Ahmed Abdelfattah; Anna Fontana; Silvia Scibetta; Sonia Pangallo
PhD students: Giuseppe Micali; Imen Belgacem
Research fellows: Ahmed Abdelfattah; Anna Fontana; Silvia Scibetta; Sonia Pangallo; Orlando Campolo; Giuseppe Massimo Algeri; Francesca Laudani; Giulia Giunti; Antonio Dattola; Vincenzo Vonella
Project-based collaborators: Anna Maria Cichello; Giovanna Leo

Principal aims

The Biotechnology laboratory carry out multidisciplinary research activities with integrated approaches. Include the following fields: Plant Defence, General and Applied Entomology, and Tree crops.
In plant defence field research activities were carried out through the monitoring and study of diseases that are already or may become a threat to crops health, in particular Mediterranean crops, including phythophthora spp. and Armillaria, olive leprosy, peacock's eye and plant virus and viroids, in particular on citrus, vine, olive and vegetables crops. Much attention is also paid to the development of alternative means of fight versus field and post-harvest diseases (fungi, bacteria and viruses), based mainly on the use of microbial populations and substances derived by vegetal and natural extracts The fight against mycotoxigenic fungi falls into this reseach line.
In general and applied entomology, the projects activated at the laboratory are aimed at identifying and studying methods with a low environmental impact for the control of pest-infesting animal crops and foodstuffs. Further, consultancy activities were carried put for the main multinational companies producing agropharmaceuticals for the verification of the effectiveness, selectivity and phytotoxicity of the new synthetic insecticidal molecules, taking care of the experimental protocols and the management of the operations entrusted to different Test Centers
In tree crops field research strategies are aimed at improving the interaction between tree, soil and atmosphere of typically Mediterranean fruit tree species, or of new introduction, researching innovations in the management of fruit trees, with particular reference to pruning, fertilization, irrigation and soil management techniques, study of "water relations", research of new germplasm and conservation of varieties in of extinction. All activities are finalized to improvement in production, both in quantitative that in qualitative aspects. With reference qualitative aspect, the attention is focus on the new needs of the market.


Research activities

Biotechnology Laboratory has been operating in the Calabrian territory for more than twenty years and is equipped with skills and equipment that allow to carry out advanced analysis:
- Biological-molecular characterization of Phythophthora spp. in agrarian and forestal ecosystems;
- Study of the genetic diversity of Phytophthora species through molecular analysis (metagenomic analysis, SSR, SNP)
- Study of the biology and ecology of causal agents of olive leprosy;
- Development of plant defense systems versus main diseases (fungi, bacteria, viruses), environmentally friendly and safe for agricultural operators and consumers. In this context different means of control are experimented under experimental and commercial conditions, including mainly plant extracts, antagonist microorganisms and organic and inorganic salts;
- Study of plant microbiome both with conventional mycological analysis tools and with advanced molecular analysis (metagenomic analysis);
- Development and application of molecular diagnostics for the diagnosis of important phytopathogenic fungi in the Mediterranean environment;
- Monitoring and characterization of the citrus fruitlessness virus;
- Diagnosis and characterization of viral agents included quarantine pathogens lists
- Application of micro-propagation and virus recovery techniques for grapevines, olive trees and main fruit-tree crops of interest for Calabrian agriculture.
- Monitoring and characterization of viruses and virus-like of grapevine
- Monitoring and characterization of olive virus.
- Monitoring and characterization of horticultural species viruses (tomato, pepper, hot pepper, onion)
- Study of plant-pathogen interactions, in particular plant viruses/vegetable crops, in the accumulation of secondary metabolites of nutraceutical interest
- Development of specific molecular diagnostic protocols (Molecular hybridization, Polymerase chain reaction-PCR, ReatlTime-PCR) for the main viral pathogens reported in Calabria and/or present in the Alert List EPPO A1 / A2
- Virological metagenomic analysis of plant samples
- Sequencing of viral genomes
- Health certification for grapevine, olive trees and other fruit trees (laboratory accreditation)
- use of natural substances for pest control;
- bio-ethological studies on the main plant and foodstuff parasites;
- development of biological and integrated control methods;
- development of attractants and repellents.
- HCCP Protocol set up and validation
- Analisi delle impurità di prodotti alimentari tramite filth-test
- Determination of maturation indexes of fruit trees: carpological parameters (weight, shape and size of fruits), organoleptic (content in solid total soluble, titratable acidity, acid / sugar ratio, content in fructose, glucose, sucrose); evaluation of the degree of coloring of the epicarp and of the skin; determination of nutraceutical parameters: total antioxidant capacity, vitamin C content, total polyphenol content and flavonoids; fat content
- Agri-food quality control
- Evaluation of the correct management of the foliage of the fruit trees and possible interbvents
- Evaluation of nutritional needs of fruit trees - Leaf diagnostics - implementation of leaflet diagnostic protocols: from the time and the sampling methods to the preparation of matrices for the determination of macro and microelements
- Analysis of impurities of food products through filth-test


International relationships

James Hutton Institute in Scozia; United States Department of Agriculture (USDA) in California e West Virginia (USA); University of Western Sydney (Australia); University of Berkeley (USA); The Volcani Center (Israel); University of California (USA); Phytophthora Research Centre (Czech Republic), IGM, Université Paris-sud (Francia), Food and Environmental Research Agency (FERA) – York (UK); Agricultural Research Organization (ARO); Ministry of Agriculture and Rural Development Israel; University of Tabriz, IRAN; University of Sfax, Faculty of Sciences, Tunisia; Italian Agricultural Research Council, Italy; Institute of Chemistry of Nice (UMR CNRS 7272), University of Nice, Sophia Antipolis, France; Institute Sophia Agrobiotech - UMR INRA 1355 CNRS 7254 UNS, France; Dipartimento di Agraria, University of Napoli “Federico II”, Italy; Institut Agronomique et Vétérinaire Hassan II - Complexe Horticole d’Agadir, Morocco; Hellenic Agricultural Organisation – Demeter, Greece; Universitat Politècnica de València, Spain


Smart Specialisation Strategy Calabria (S3 Calabria)

Agroalimentare

Research fields

Responsible Keywords ERC Domain
Gaetano Magnano Di San Lio LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Giovanni Enrico Agosteo LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Leonardo Schena LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Giuliana Renata Albanese LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Vincenzo Palmeri Stored product protection, Thermal remediation, Filth-test LS9_3 - Agriculture related to animal husbandry, dairying, livestock raising
LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
-
Vincenzo Palmeri Insecticides, natural extracts, selectivity LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Vincenzo Palmeri Beekeping, animal parasites, pollinators LS9_3 - Agriculture related to animal husbandry, dairying, livestock raising
Vincenzo Palmeri Biological control, beneficials rearing, behaviour. LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Rocco Zappia Valorization of vitis germplasm; Improvement of the management techniques of olive, vine, citrus, and fruiting plants LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Maria Giulia Li Destri Nicosia LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Gregorio Antonio Maria Gullo Strategies for the improvement of physiological and vegetative andproductive performance of fruit tree: olive, citrus, vine and fruiting (actinidia, peach); LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Rocco Mafrica Research and conservation of germplasm: olive and fruit trees LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Valentino Branca improvement of olive and citrus cultivation techniques LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology
Antonio Tiberini LS9_5 - Agriculture related to crop production, soil biology and cultivation, applied plant biology

Outsourcing

Responsible Keywords Services
Vincenzo Palmeri Effectiveness, selectivity, vulnerability windows, insecticides Field and laboratory tests to assess efficacy, selectivity and timing of treatment with insecticides. Plan development for the stored product pest management
Vincenzo Palmeri Pest control, pest management, food products Plan development for the stored product pest management
Vincenzo Palmeri Side effects, toxicity, natural enelies Evaluation of the selectivity of insecticides towards non-target organisms
Leonardo Schena Identification of phytopathogens Phytopathogenic agents identification
Leonardo Schena Pest control, plant defense Development of bio-formualte based on vegetal extract to contrast plant diseases
Giuliana Renata Albanese Plant Virology Sanitary certification for grapevine, olive, and fruit-tree
Giuliana Renata Albanese Plant Virology Citrus tristeza virus (CTV) Diagnosis

Great equipment

Attrezzatura:
  • 1 Dispositivo Purificazione per Acqua Ultrapura

  • 1 Congelatore (-20°C)

  • 1 Liofilizzatore

  • 1 Centrifuga da laboratorio con rotore basculante

  • 1 Centrifuga da banco tubi 2ml

  • 1 Essiccatore a centrifuga sotto vuoto

  • 1 Stereomicroscopio ottico

  • 1 Bilancia di Precisione

  • 1 Cella di crescita climatica

  • 1 Spettrofotometro Nanodrop

  • 1 RealTime StepOne plus

  • 1 Fornetto Ibridazione

Responsabili Leonardo Schena
Classificazione: Environmental Sciences;
Fondi: POR Calabria FESR 2007-2013 misura 1.1.1.1 “Biotecnologie e sistemi innovativi per le produzioni agrarie
Utenza: Interna
Applicazioni: Progetti di ricerca e contratti di ricerca

Attrezzatura: Liofilizzatore
Responsabili Leonardo Schena
Classificazione: Environmental Sciences
Fondi: PON03PE_00090
Utenza: Interna
Applicazioni: Progetti di ricerca e contratti di ricerca

Attrezzatura:
  • 1 Macchina di produzione per il ghiaccio

  • 2 Incubatori termici

  • 1 Frigorifero (4 °C)

  • 1 Cappa Chimica aspirante

  • 1 Microcentrifuga da banco per tubi da 1,5/2 ml

  • 1 Microscopio a fluorescenza Olympus FSX100

  • 3 Microscopi ottici a immersione

  • 1 Cappa a flusso Laminare

  • 3 Autoclave da banco

  • 1 Termociclatori per PCR convenzionale
Responsabili Leonardo Schena
Classificazione: Environmental Sciences
Fondi:
Utenza: Interna
Applicazioni: Progetti di ricerca e contratti di ricerca

Attrezzatura: 1 Cella di crescita climatica
Responsabili Leonardo Schena
Classificazione: Environmental Sciences
Fondi: FEARS 2007_2013 "Tecniche innovative per il miglioramento sanitario del germoplasma vitivolo autoctono Calabrese"
Utenza: Interna
Applicazioni: Progetti di ricerca e contatti di ricerca

Attrezzatura: 1 Sequenziatore NGS Illumina MiSeq
Responsabili Leonardo Schena
Classificazione: Environmental Sciences
Fondi: PON03PE_00090
Utenza: Interna
Applicazioni: Progetti di ricerca e contratti di ricerca

Salita Melissari - 89124 Reggio Calabria - CF 80006510806 - Fax 0965 332201 - URP:Indirizzo di posta elettronica dell'ufficio relazioni con il pubblico- PEC:Indirizzo di posta elettronica certificata dell'amministrazione
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